SANTA CRUZ BIOTECHNOLOGY, INC.
CD223 (C9B7W): sc-32750
The Power to Question
BACKGROUND
CD223 (Lymphocyte activation gene-3, LAG-3) is a high affinity MHC class II
ligand present on the surface of CD4
+
/CD8
+
T cells and NK cells. CD223
shares homology in structure to CD4 molecules, having four similar extracel-
lular Ig-like domains and structural motifs between D1-D3 and D2-D4
domains. CD223 has a glutamic acid-proline (EP) repetitive sequence found
in other functionally distinct mammalian, parasitic, and bacterial proteins
that may influence a conserved biological function. CD223
+
/CD4
+
/CD8
+
T
cells can associate with the T cell receptor (TCR) and downregulate TCR sig-
naling
in vitro.
CD223 inhibits CD4-dependent T cell function via its cytoplas-
mic domain . CD223 Lys-468 within a conserved "KIEELE" motif is essential
for interaction with downstream signaling molecules.
APPLICATIONS
CD223 (C9B7W) is recommended for detection of CD223 of mouse origin by
flow cytometry (1 µg per 1 x 10
6
cells).
Suitable for use as control antibody for CD223 siRNA (m): sc-44548.
Molecular Weight of CD223: 70 kDa.
DATA
REFERENCES
1. Demeure, C.E., et al. 2001. T Lymphocytes infiltrating various tumour
types express the MHC class II ligand lymphocyte activation gene-3 (LAG-
3): role of LAG-3/MHC class II interactions in cell-cell contacts. Eur. J.
Cancer 37: 1709-1718.
2. Online Mendelian Inheritance in Man, OMIM™. 2002. Johns Hopkins
University, Baltimore, MD. MIM Number: 153337. World Wide Web URL:
http://www.ncbi.nlm.nih.gov/omim/
3. LocusLink Report (LocusID: 3902). http://www.ncbi.nlm.nih.gov/LocusLink/
4. Andreae, S., et al. 2002. Maturation and activation of dendritic cells
induced by lymphocyte activation gene-3 (CD223). J. Immunol. 168:
3874-3880.
5. Workman, C.J., et al. 2002. Phenotypic analysis of the murine CD4-related
glycoprotein, CD223 (LAG-3). Eur. J. Immunol. 32: 2255-2263.
6. Workman, C.J., et al. 2002. Cutting edge: molecular analysis of the nega-
tive regulatory function of lymphocyte activation gene-3. J. Immunol. 169:
5392-5395.
7. Buisson, S., et al. 2003. MHC class II engagement by its ligand LAG-3
(CD223) leads to a distinct pattern of chemokine and chemokine receptor
expression by human dendritic cells. Vaccine 21: 862-868.
CD223 (C9B7W) PE: sc-32750 PE. FCM analysis of
CTLL-2 cells. Black line histogram represents the iso-
type control, normal rat IgG1: sc-2871.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
PROTOCOLS
See our web site at www.scbt.com or our catalog for detailed protocols
and support products.
CHROMOSOMAL LOCATION
Genetic locus: LAG3 (human) mapping to 12p13.3; LAG3 (mouse) mapping to 6.
SOURCE
CD223 (C9B7W) is a rat monoclonal antibody raised against a fusion protein
consisting of the entire extracellular region of mouse CD223 with mouse
IgG
1
and recognizes an epitope in the D2 domain of CD223.
PRODUCT
Each vial contains 200 µg IgG
1
in 1.0 ml of PBS with < 0.1% sodium azide
and 0.1% gelatin.
Available as phycoerythrin (sc-32750 PE) or fluorescein (sc-32750 FITC)
conjugates for flow cytometry, 100 tests.
Santa Cruz Biotechnology, Inc.
1.800.457.3801
831.457.3800
fax 831.457.3801
Europe
+00800 4573 8000
49 6221 4503 0
www.scbt.com
