以一株耐高渗透压的酵母菌种作为敏感材料, 研制的的生物传感器可采用稳态呼吸速率法进行BOD 快速测定。该方法与广为采用的五天生化需氧量标准稀释测定法有良好的相关性, 线性范围为0～ 200mgöL , 可在15m in 内完成一个样品BOD 的测定。测定结果具有良好的重现性和准确度, 标准曲线的线性相关系数达到019987。关　键　词:BOD; 生物传感器; 酵母细胞Abstract: A biochem ical oxygen demand (BOD) senso r, p repared from a st rain of o smo to lerant yeast, is calibrated by determ ining the steady2state rate of its response in various standard gluco seög lutam ic acid BOD2 check so lut ions1 The steady2state rate varies linearly w ith the five2day BOD values from 0 to 200 mgöL 1 Typ ical response t imes of the BOD senso r w ere 5～ 15m in1 The results show good recurrence and specificity, and the co rrelat ion coefficient of the calibra2 t ion curve w ere at 0199871Key words: BOD; bio senso r; yeast